ELISA binding curve of biotinylated human EPOR with hFc-tagged EPO, showing mean absorbance (OD450) across increasing EPO concentrations.
Biotinylated Human EPO R/Erythropoietin R Protein (EPO-HM50RB)
  • Express System: HEK293
  • Product Tag: C-hFc-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human EPO, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human EPO R, hFc Tag with the EC50 of 0.70ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ala25-Pro250
Catalog #EPO-HM50RB
Regular price $577.50
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Product Description

Recombinant Biotinylated Human EPO R/Erythropoietin R Protein is expressed from HEK293 with hFc tag and Avi tag at the C-terminus.It contains Ala25-Pro250.

Product Background

The erythropoietin (epo) receptor is a member of the cytokine receptor family. It is expressed almost exclusively on erythroid precursor cells and controls the development of red blood cells. The epo receptor has no intrinsic kinase activity, but binds intracellular tyrosine kinases to elicit its signals. Alterations in the transmission of the signalling cascade lead to clinically abnormal red blood cell production.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others
Protein
EPO R/Erythropoietin R
Synonyms
EPO-R; Erythropoietin R; EPO R; EPOR
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Ala25-Pro250
Molecular Weight
The protein has a predicted MW of 52.58 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human EPO, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human EPO R, hFc Tag with the EC50 of 0.70ug/ml determined by ELISA. See testing image for detail.

Product Tag

C-hFc-Avi
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human EPO, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human EPO R, hFc Tag with the EC50 of 0.70ug/ml determined by ELISA. Immobilized Human EPO, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human EPO R, hFc Tag with the EC50 of 0.70ug/ml determined by ELISA.
Biotinylated Human EPO R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human EPO R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human EPO R is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human EPO R is greater than 95% as determined by SEC-HPLC.
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