ELISA binding curve showing the interaction between biotinylated Human FcRH5 (His Tag) and anti-FcRH5 antibody (hFc Tag), demonstrating a dose-dependent increase in absorbance indicative of specific binding affinity.
Biotinylated Human FcRH5/FcRL5 Protein (FCR-HM401B)
Biotinylated Human FcRH5/FcRL5 Protein (FCR-HM401B)
Biotinylated Human FcRH5/FcRL5 Protein (FCR-HM401B)
  • Express System: HEK293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Anti-FcRH5 Antibody at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FcRH5, His Tag with the EC50 of 93.6ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gln16-Gly851
Catalog #FCR-HM401B
Regular price $742.50
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Product Description

Recombinant Biotinylated Human FcRH5/FcRL5 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Gln16-Gly851.

Product Background

FcRH5 is a cell surface marker enriched on malignant plasma cells when compared to other hematologic malignancies and normal tissues. DFRF4539A is an anti-FcRH5 antibody-drug conjugated to monomethyl auristatin E (MMAE), a potent anti-mitotic agent.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others
Protein
FcRH5/FcRL5
Synonyms
FcR-like protein 5; FcRH5; FcRL5; BXMAS1; BXMAS1MGC119592; CD307; CD307e; DKFZp667E2019; DKFZp667F216; FCRH5MGC119593; FLJ00333; FLJ00397; IFGP5; IRTA2
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Gln16-Gly851
Molecular Weight
The protein has a predicted MW of 94.19 kDa. Due to glycosylation, the protein migrates to 90-130 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Anti-FcRH5 Antibody at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FcRH5, His Tag with the EC50 of 93.6ng/ml determined by ELISA. See testing image for detail.

Product Tag

N-His-Avi
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Anti-FcRH5 Antibody at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FcRH5, His Tag with the EC50 of 93.6ng/ml determined by ELISA. Immobilized Anti-FcRH5 Antibody at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FcRH5, His Tag with the EC50 of 93.6ng/ml determined by ELISA.
Biotinylated Human FcRH5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human FcRH5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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