Name: Biotinylated Human FcRH5/FcRL5 Protein (FCR-HM401B)
Brand: KactusBio
SKU: FCR-HM401B-100UG
Price: 742.5 USD
Availability: InStock

ELISA binding curve showing the interaction between biotinylated Human FcRH5 (His Tag) and anti-FcRH5 antibody (hFc Tag), demonstrating a dose-dependent increase in absorbance indicative of specific binding affinity.

Biotinylated Human FcRH5/FcRL5 Protein (FCR-HM401B)

Immobilized Biotinylated Human FcRH5, His Avi Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-FcRH5 Antibody, hFc Tag with the EC50 of 20.9ng/ml determined by ELISA.

Biotinylated Human FcRH5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Loaded Anti-FcRH5 Ab., hFc Tag on ProA-Biosensor can bind Biotinylated Human FcRH5, His Tag with an affinity constant of 13.70 nM as determined in BLI assay (Gator® Prime).

Express System: HEK293
Product Tag: N-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Biotinylated Human FcRH5, His Avi Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-FcRH5 Antibody, hFc Tag with the EC50 of 20.9ng/ml determined by ELISA.
Amino Acid Range: Gln16-Gly851
Other Available Proteins:

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Catalog #FCR-HM401B

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human FcRH5/FcRL5 Protein (FCR-HM401B) Summary

Product Description	Recombinant Biotinylated Human FcRH5/FcRL5 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Gln16-Gly851.
Product Background	FcRH5 is a cell surface marker enriched on malignant plasma cells when compared to other hematologic malignancies and normal tissues. DFRF4539A is an anti-FcRH5 antibody-drug conjugated to monomethyl auristatin E (MMAE), a potent anti-mitotic agent.
Product Category	Recombinant Protein / Antibody discovery / ADC targets

Biotinylated Human FcRH5/FcRL5 Protein (FCR-HM401B) Specifications/Details

Protein	FcRH5/FcRL5
Synonyms	FcR-like protein 5; FcRH5; FcRL5; BXMAS1; BXMAS1MGC119592; CD307; CD307e; DKFZp667E2019; DKFZp667F216; FCRH5MGC119593; FLJ00333; FLJ00397; IFGP5; IRTA2
Accession	Q96RD9-1
Species	Human
Biotinylated	yes
Amino Acid Range	Gln16-Gly851
Molecular Weight	The protein has a predicted MW of 94.19 kDa. Due to glycosylation, the protein migrates to 90-130 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human FcRH5, His Avi Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-FcRH5 Antibody, hFc Tag with the EC50 of 20.9ng/ml determined by ELISA.
Product Tag	N-His-Avi
Fluorophore	Biotin-labeled
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Biotinylated Human FcRH5, His Avi Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-FcRH5 Antibody, hFc Tag with the EC50 of 20.9ng/ml determined by ELISA.
	Biotinylated Human FcRH5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Anti-FcRH5 Ab., hFc Tag on ProA-Biosensor can bind Biotinylated Human FcRH5, His Tag with an affinity constant of 13.70 nM as determined in BLI assay (Gator® Prime).

Biotinylated Human FcRH5/FcRL5 Protein (FCR-HM401B) Summary

Product Description

Product Background

Product Category

Biotinylated Human FcRH5/FcRL5 Protein (FCR-HM401B) Specifications/Details

Fluorophore

Shipping, Reconstitution, & Storage

Validation Images

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