Biotinylated Human IL-1 Rrp2/IL-1 R6 Protein (IL1-HM4L2B)
Biotinylated Human IL-1 Rrp2/IL-1 R6 Protein (IL1-HM4L2B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human IL-1 Rrp2, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-IL-1 Rrp2 Antibody, hFc Tag with the EC50 of 6.1ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Asp20-Arg335
Catalog #IL1-HM4L2B
Regular price $525.00
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Product Description

Recombinant Biotinylated Human IL-1 Rrp2/IL-1 R6 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp20-Arg335.

Product Background

The Interleukin 1 receptor family (IL-1 R) comprises at least eleven members including IL-1 RI (IL-1 R1), IL-1 RII (IL-1 R2), IL-1 RAcP (IL‑1 R3), ST2 (T1/IL-1 R4), IL-18 Ra (IL-1 Rrp/IL-1 R5), IL-1 Rrp2 (IL-1 RL2/IL-1 R6), IL-18 Rb (AcPL/IL-1 R7), IL-1RAPL‑1 (TIGIRR‑2/IL‑1 R8), and TIGIRR-1 (IL-1 R9). IL1RL2 is a receptor for interleukin-36 (IL36A, IL36B and IL36G). After binding to interleukin-36 associates with the coreceptor IL1RAP to form the interleukin-36 receptor complex which mediates interleukin-36-dependent activation of NF-kappa-B, MAPK and other pathways.

Product Category

Recombinant Protein / Antibody discovery

Protein

IL-1 Rrp2/IL-1 R6

Synonyms

IL-1 R6; IL-1 Rrp2; IL1RL2; IL1Rrp2; IL1RRP2; IL-36R

Accession

Species

Human

Biotinylated

yes

Amino Acid Range

Asp20-Arg335

Molecular Weight

The protein has a predicted MW of 39.1 kDa. Due to glycosylation, the protein migrates to 55-75 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Biotinylated Human IL-1 Rrp2, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-IL-1 Rrp2 Antibody, hFc Tag with the EC50 of 6.1ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human IL-1 Rrp2, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-IL-1 Rrp2 Antibody, hFc Tag with the EC50 of 6.1ng/ml determined by ELISA (QC Test). Immobilized Biotinylated Human IL-1 Rrp2, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-IL-1 Rrp2 Antibody, hFc Tag with the EC50 of 6.1ng/ml determined by ELISA (QC Test).
Biotinylated Human IL-1 Rrp2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human IL-1 Rrp2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human IL-1 Rrp2 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human IL-1 Rrp2 is greater than 95% as determined by SEC-HPLC.

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