Name: Biotinylated Human IL-10 Protein (IL1-HM410B)
Brand: KactusBio
SKU: IL1-HM410B-100UG
Price: 660.0 USD
Availability: InStock

Biotinylated Human IL-10 Protein (IL1-HM410B)

Human IL-10 R alpha, His Tag immobilized on CM5 Chip can bind Biotinylated Human IL-10, His Tag with an affinity constant of 0.60 nM as determined in SPR assay (Biacore T200).

Biotinylated Human IL-10 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Express System: HEK293
Product Tag: N-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE
Activity: The affinity constant of 0.60 nM as determined in SPR assay (Biacore T200).
Amino Acid Range: Ser19-Asn178

Catalog #IL1-HM410B

Size

100UG

500UG

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Biotinylated Human IL-10 Protein (IL1-HM410B) Summary

Product Description	Recombinant Biotinylated Human IL-10 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Ser19-Asn178.
Product Background	Interleukin 10 (IL10) is a key anti-inflammatory cytokine that can inhibit proinflammatory responses of both innate and adaptive immune cells. An association between IL10 and intestinal mucosal homeostasis became clear with the discovery that IL10 and IL10 receptor (IL10R)-deficient mice develop spontaneous intestinal inflammation. Similarly, patients with deleterious mutations in IL10, IL10RA, or IL10RB present with severe enterocolitis within the first months of life.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Biotinylated Human IL-10 Protein (IL1-HM410B) Specifications/Details

Protein	IL-10
Synonyms	Interleukin-10; IL-10; CSIF; IL10A; TGIF; B-TCGF; GVHDS; MGC126450; MGC126451
Accession	P22301
Species	Human
Biotinylated	yes
Amino Acid Range	Ser19-Asn178
Molecular Weight	The protein has a predicted MW of 21.3 kDa. Due to glycosylation, the protein migrates to 23-25 kDa based on Bis-Tris PAGE result.
Activity	The affinity constant of 0.60 nM as determined in SPR assay (Biacore T200).
Product Tag	N-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Human IL-10 R alpha, His Tag immobilized on CM5 Chip can bind Biotinylated Human IL-10, His Tag with an affinity constant of 0.60 nM as determined in SPR assay (Biacore T200).
	Biotinylated Human IL-10 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.