Biotinylated Human IL-25/IL-17E Protein (IL2-HM425B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Human IL-17RB, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-25, His Tag with the EC50 of 58.5ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Tyr33-Gly177

Catalog: IL2-HM425B

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Product Description Recombinant Biotinylated Human IL-25/IL-17E Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Tyr33-Gly177.

Product Background

IL-25, also known as IL-17E, is a member of the IL-17 cytokine family mostly produced by epithelial cells and innate immune cells. After binding to the IL-17RB/IL-17RA complex, IL-25 induces downstream signaling responses in epithelial cells and type 2 lymphocytes, which initiates, propagates, and sustains type 2 immunity. The function of IL-25 in allergic diseases such as asthma has been well established, and now also is extended to diseases such as inflammatory bowel disease and cancer.

Product Category

Recombinant Protein

Target

IL-25/IL-17E
Synonyms IL-25; IL-17E; IL17E; Interleukin-17E; interleukin-25

Gene/Protein ID

Q9H293-1

Species

Human

Exact Sequence

Tyr33-Gly177
Molecular Weight The protein has a predicted MW of 19.24 kDa. Due to glycosylation, the protein migrates to 25-35 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human IL-17RB, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-25, His Tag with the EC50 of 58.5ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human IL-17RB, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-25, His Tag with the EC50 of 58.5ng/ml determined by ELISA.
The purity of Biotinylated Human IL-25 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human IL-25 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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