Biotinylated Human IL-25/IL-17E Protein (IL2-HM425B)
Catalog: IL2-HM425B
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Product Description | Recombinant Biotinylated Human IL-25/IL-17E Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Tyr33-Gly177. |
Product Background |
IL-25, also known as IL-17E, is a member of the IL-17 cytokine family mostly produced by epithelial cells and innate immune cells. After binding to the IL-17RB/IL-17RA complex, IL-25 induces downstream signaling responses in epithelial cells and type 2 lymphocytes, which initiates, propagates, and sustains type 2 immunity. The function of IL-25 in allergic diseases such as asthma has been well established, and now also is extended to diseases such as inflammatory bowel disease and cancer. |
Product Category |
Recombinant Protein |
Target |
IL-25/IL-17E |
Synonyms | IL-25; IL-17E; IL17E; Interleukin-17E; interleukin-25 |
Accession | |
Species |
Human |
Amino Acid Range |
Tyr33-Gly177 |
Molecular Weight | The protein has a predicted MW of 19.24 kDa. Due to glycosylation, the protein migrates to 25-35 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Human IL-17RB, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-25, His Tag with the EC50 of 58.5ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 1EU per ug by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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