Name: Biotinylated Human IL-25/IL-17E Protein (IL2-HM425B)
Brand: KactusBio
SKU: IL2-HM425B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human IL-25/IL-17E Protein (IL2-HM425B)

Immobilized Human IL-17RB, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-25, His Tag with the EC50 of 58.5ng/ml determined by ELISA.

Biotinylated Human IL-25 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human IL-25 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IL-17RB, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-25, His Tag with the EC50 of 58.5ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Tyr33-Gly177

Catalog #IL2-HM425B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human IL-25/IL-17E Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Tyr33-Gly177.
Product Background	IL-25, also known as IL-17E, is a member of the IL-17 cytokine family mostly produced by epithelial cells and innate immune cells. After binding to the IL-17RB/IL-17RA complex, IL-25 induces downstream signaling responses in epithelial cells and type 2 lymphocytes, which initiates, propagates, and sustains type 2 immunity. The function of IL-25 in allergic diseases such as asthma has been well established, and now also is extended to diseases such as inflammatory bowel disease and cancer.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	IL-25/IL-17E
Synonyms	IL-25; IL-17E; IL17E; Interleukin-17E; interleukin-25
Accession	Q9H293-1
Species	Human
Biotinylated	yes
Amino Acid Range	Tyr33-Gly177
Molecular Weight	The protein has a predicted MW of 19.24 kDa. Due to glycosylation, the protein migrates to 25-35 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human IL-17RB, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-25, His Tag with the EC50 of 58.5ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human IL-17RB, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-25, His Tag with the EC50 of 58.5ng/ml determined by ELISA.
	Biotinylated Human IL-25 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human IL-25 is greater than 95% as determined by SEC-HPLC.

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