Product Description |
Recombinant Biotinylated Human LAP (TGF beta 1) Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Leu30-Arg278(C33S). |
Product Background |
Latent TGF beta 1 cDNA encodes a 390 amino acid precursor that contains a 29 aa signal peptide and a 361 aa proprotein. A furin‑like convertase processes the proprotein to generate an N‑terminal 249 aa latency‑associated peptide (LAP) and a C‑terminal 112 aa mature TGF‑ beta 1. Disulfide‑linked homodimers of LAP and TGF‑ beta 1 remain non‑covalently associated after secretion, forming the small latent TGF‑ beta 1 complex. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Protein |
LAP (TGF beta 1) |
Synonyms |
CEDLAP; DPD1; TGF beta1; TGFB; TGFB1; TGFbeta; TGF-beta-1; TGF β1; TGFβ; TGF-β-1; TGF-beta 1 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Leu30-Arg278(C33S) |
Molecular Weight |
The protein has a predicted MW of 31.4 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human ITGAV&ITGB6, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human LAP (TGF beta 1) , His Tag with the EC50 of 0.11ug/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
N-His-Avi |
Expression System |
HEK293 |
Purity |
> 90% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Immobilized Human ITGAV&ITGB6, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human LAP (TGF beta 1) , His Tag with the EC50 of 0.11ug/ml determined by ELISA (QC Test). |
|
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Biotinylated Human LAP (TGF beta 1) on Bis-Tris PAGE under reduced condition. The purity is greater than 90%. |
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