Biotinylated Human LILRB3/CD85a/ILT5 Protein (LIL-HM4B3B)
Biotinylated Human LILRB3/CD85a/ILT5 Protein (LIL-HM4B3B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human LILRB3, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-LILRB3 Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gly24-Glu443
Catalog #LIL-HM4B3B
Regular price $577.50
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Product Description

Recombinant Biotinylated Human LILRB3/CD85a/ILT5 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly24-Glu443.

Product Background

Leukocyte immunoglobulin-like receptor subfamily B (LILRB3), also known as ILT5, LIR3, and CD85a, is an immunoglobulin superfamily member that is involved in immune regulation. Subfamily B members have cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that inhibit signaling events via phosphatase SHP-1.LILRB3 may act as receptor for class I MHC antigens. Becomes activated upon coligation of LILRB3 and immune receptors, such as FCGR2B and the B-cell receptor.

Product Category

Recombinant Protein / Antibody discovery / Immune checkpoint proteins
Protein
LILRB3/CD85a/ILT5
Synonyms
CD85a; ILT5; ILT-5; LILRB3; LIR3; LIR3CD85A; LIR-3MGC138403; PIRB; HL9
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Gly24-Glu443
Molecular Weight
The protein has a predicted MW of 50 kDa. Due to glycosylation, the protein migrates to 60-72 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human LILRB3, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-LILRB3 Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human LILRB3, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-LILRB3 Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA. Immobilized Biotinylated Human LILRB3, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-LILRB3 Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA.
Biotinylated Human LILRB3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human LILRB3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human LILRB3 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human LILRB3 is greater than 95% as determined by SEC-HPLC.
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