Biotinylated Human NCAM-1/CD56 Protein (CAM-HM456B)
Biotinylated Human NCAM-1/CD56 Protein (CAM-HM456B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human NCAM-1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-NCAM-1 Antibody, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Leu20-Gly718
Catalog #CAM-HM456B
Regular price $577.50
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Product Description

Recombinant Biotinylated Human NCAM-1/CD56 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Leu20-Gly718.

Product Background

Neural Cell Adhesion Molecule 1 (NCAM-1), a multifunctional member of the immunoglobulin superfamily, is expressed on the surface of neurons, glia, skeletal muscle, and natural killer cells. NCAM-1 has been implicated as having a role in cell-cell adhesion, involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others
Protein
NCAM-1/CD56
Synonyms
N-CAM-1; NCAM-1; NCAM1; CD56; NCAM; MSK39
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Leu20-Gly718
Molecular Weight
The protein has a predicted MW of 80.29 kDa. Due to glycosylation, the protein migrates to 100-130 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human NCAM-1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-NCAM-1 Antibody, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human NCAM-1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-NCAM-1 Antibody, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA. Immobilized Biotinylated Human NCAM-1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-NCAM-1 Antibody, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA.
Biotinylated Human NCAM-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human NCAM-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human NCAM-1 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human NCAM-1 is greater than 95% as determined by SEC-HPLC.
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