Name: Biotinylated Human Nectin-3/CD113 Protein (NEC-HM403B)
Brand: KactusBio
SKU: NEC-HM403B-100UG
Price: 577.5 USD
Availability: InStock

Binding affinity curve showing response units (RU) versus analyte concentration for KD determination in SPR analysis.

Biotinylated Human Nectin-3, His Tag immobilized on CM5 Chip can bind Human Nectin-1, His Tag with an affinity constant of 0.13 uM as determined in SPR assay (Biacore T200).

Immobilized Biotinylated Human Nectin-3, His-Avi Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human Nectin-1, hFc Tag with the EC50 of 3.1ng/ml determined by ELISA (QC Test).

Biotinylated Human Nectin-3 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

The purity of Biotinylated Human Nectin-3 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human Nectin-3, His-Avi Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human Nectin-1, hFc Tag with the EC50 of 3.1ng/ml determined by ELISA (QC Test). The affinity constant of 0.13 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Gly58-Asp400

Catalog #NEC-HM403B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human Nectin-3/CD113 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly58-Asp400.
Product Background	The Nectin family has at least four members (Nectin-1‑4), all of which show alternate splicing, a transmembrane (TM) region (except for Nectin-1 gamma), and three extracellular Ig-domains. Nectins are highly homologous to the human receptor for poliovirus, and as such, have been alternatively-named poliovirus receptor-related proteins. They do not, however, appear to bind poliovirus. Nectin-3 (also named PRR3, CD113, and PVRL3) is an 83 kDa, type I TM glycoprotein.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein	Nectin-3/CD113
Synonyms	CD113; CDw113; FLJ90624; PPR3; PVRL3; PVRR3; PRR3
Accession	Q9NQS3-1
Species	Human
Biotinylated	yes
Amino Acid Range	Gly58-Asp400
Molecular Weight	The protein has a predicted MW of 40.6 kDa. Due to glycosylation, the protein migrates to 68-82 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human Nectin-3, His-Avi Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human Nectin-1, hFc Tag with the EC50 of 3.1ng/ml determined by ELISA (QC Test). The affinity constant of 0.13 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 20mM PB, 250mM NaCl (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Please refer to the Certificate of Analysis for detailed instructions.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Biotinylated Human Nectin-3, His Tag immobilized on CM5 Chip can bind Human Nectin-1, His Tag with an affinity constant of 0.13 uM as determined in SPR assay (Biacore T200).
	Immobilized Biotinylated Human Nectin-3, His-Avi Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human Nectin-1, hFc Tag with the EC50 of 3.1ng/ml determined by ELISA (QC Test).
	Biotinylated Human Nectin-3 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
	The purity of Biotinylated Human Nectin-3 is greater than 95% as determined by SEC-HPLC.

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