Biotinylated Human NRG1 Beta 1 Protein (Primary Amine Labeling) (NRG-HM201B)
Biotinylated Human NRG1 Beta 1 Protein (Primary Amine Labeling) (NRG-HM201B)
Biotinylated Human NRG1 Beta 1 Protein (Primary Amine Labeling) (NRG-HM201B)
  • Express System: HEK293
  • Product Tag: N-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Her4, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human NRG1 Beta 1, hFc Tag with the EC50 of 0.65ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ser2-Lys246
Catalog #NRG-HM201B
Regular price $577.50
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Product Description

Recombinant Biotinylated Human NRG1 Beta 1 Protein (Primary Amine Labeling) is expressed from HEK293 with hFc tag at the N-terminus.It contains Ser2-Lys246.

Product Background

Neuregulin-1 (NRG-1) is a ligand of the epidermal growth factor receptor (erbB), and its interaction involves activation of the glutamatergic N-methyl-Daspartate receptor, which increases the expression of the β2 subunit of the γ- aminobutyric acid receptor and subunits of the nicotinic acetylcholine receptor.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others
Protein
NRG1 Beta 1
Synonyms
Pro-NRG1; NRG1; GGF; HGL; HRGA; NDF; SMDF; Beta1; Beta1A
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Ser2-Lys246
Molecular Weight
The protein has a predicted MW of 54.00 kDa. Due to glycosylation, the protein migrates to 75-90 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human Her4, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human NRG1 Beta 1, hFc Tag with the EC50 of 0.65ug/ml determined by ELISA. See testing image for detail.

Product Tag

N-hFc
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human Her4, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human NRG1 Beta 1, hFc Tag with the EC50 of 0.65ug/ml determined by ELISA. Immobilized Human Her4, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human NRG1 Beta 1, hFc Tag with the EC50 of 0.65ug/ml determined by ELISA.
Biotinylated Human NRG1 Beta 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human NRG1 Beta 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human NRG1 Beta 1 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human NRG1 Beta 1 is greater than 95% as determined by SEC-HPLC.
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