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Biotinylated Human PTK7/CCK4 Protein (CCK-HM404B) Summary
Product Description |
Recombinant Biotinylated Human PTK7/CCK4 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Ala31-Thr704. |
Product Background |
Protein Tyrosine Kinase 7 (PTK7) is as a critical regulator of canonical and non-canonical Wnt-signaling during embryonic development and cancer cell formation. Disrupting PTK7 activity perturbs vertebrate nervous system development, and also promotes human cancer formation. Observations in different model systems suggest a complex cross-talk between PTK7 protein and Wnt signaling. |
Product Category |
Recombinant Protein / Antibody discovery / ADC targets |
Biotinylated Human PTK7/CCK4 Protein (CCK-HM404B) Specifications/Details
Protein |
PTK7/CCK4 |
Synonyms |
CCK-4; PTK7 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Ala31-Thr704 |
Molecular Weight |
The protein has a predicted MW of 77.52 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human PTK7, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-PTK7 Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA. |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.； -80°C for 3 months after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Biotinylated Human PTK7, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-PTK7 Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA. |
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Biotinylated Human PTK7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human PTK7 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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