Biotinylated Human ULBP-1 Protein (ULB-HM4P1B)
Biotinylated Human ULBP-1 Protein (ULB-HM4P1B)
Biotinylated Human ULBP-1 Protein (ULB-HM4P1B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human NKG2D, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ULBP-1, His Tag with the EC50 of 0.14ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gly26-Pro215
Catalog #ULB-HM4P1B
Regular price $577.50
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Product Description

Recombinant Biotinylated Human ULBP-1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly26-Pro215.

Product Background

Cell surface proteins major histocompatibility complex (MHC) class I-related chain A (MICA) and UL16-binding proteins (ULBP) 1, 2, and 3 are up-regulated upon infection or tumor transformation and can activate human natural killer (NK) cells.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others
Protein
ULBP-1
Synonyms
ALCAN-beta; NKG2D ligand 1; N2DL-1; NKG2DL1; MULT-1; NKG2DL; AET1I; ULBP1; ULBP-1;ALCAN-β
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Gly26-Pro215
Molecular Weight
The protein has a predicted MW of 25.2 kDa. Due to glycosylation, the protein migrates to 28-35 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human NKG2D, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ULBP-1, His Tag with the EC50 of 0.14ug/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in 20mM PB, 0.5M NaCl, 0.1M L-arginine (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human NKG2D, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ULBP-1, His Tag with the EC50 of 0.14ug/ml determined by ELISA. Immobilized Human NKG2D, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ULBP-1, His Tag with the EC50 of 0.14ug/ml determined by ELISA.
Biotinylated Human ULBP-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human ULBP-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human ULBP-1 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human ULBP-1 is greater than 95% as determined by SEC-HPLC.
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