Name: Biotinylated Human ULBP-2 Protein (ULB-HM402B)
Brand: KactusBio
SKU: ULB-HM402B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Human ULBP-2 Protein (ULB-HM402B)

Biotinylated Human ULBP-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Serial dilutions of Anti-NKG2D Antibody, hFc Tag were added into Biotinylated Human ULBP-2, His Tag : Human NKG2D, hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 45.2ng/ml.

The purity of Biotinylated Human ULBP-2 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human ULBP-2, His-Avi Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.30ug/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Gly26-Ser217

Catalog #ULB-HM402B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human ULBP-2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly26-Ser217.
Product Background	ULBP2 expression was however linked to expression of mature CD57() NK cells. In particular, expression of ULBP2 was strongest on those NK cells that had evidence of recent activation and proliferation. We suggest that ULBP2 could be used to identify recently activated "mature" NK cells.
Product Category	Recombinant Protein

Protein	ULBP-2
Synonyms	ALCAN-alpha; N2DL2; NKG2DL2; RAET1H; RAET1L; ULBP2; ULBP-2/5/6; NKG2D ligand 2
Accession	Q9BZM5
Species	Human
Biotinylated	yes
Amino Acid Range	Gly26-Ser217
Molecular Weight	The protein has a predicted MW of 24.6 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human ULBP-2, His-Avi Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.30ug/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Biotinylated Human ULBP-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Serial dilutions of Anti-NKG2D Antibody, hFc Tag were added into Biotinylated Human ULBP-2, His Tag : Human NKG2D, hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 45.2ng/ml.
	The purity of Biotinylated Human ULBP-2 is greater than 95% as determined by SEC-HPLC.