Biotinylated Human ULBP-2 Protein (ULB-HM402B)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human ULBP-2, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.2μg/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gly26-Ser217

Catalog: ULB-HM402B

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Product Description Recombinant Biotinylated Human ULBP-2 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Gly26-Ser217.
Product Category Recombinant Protein
Target ULBP-2
Synonyms ALCAN-alpha; N2DL2; NKG2DL2; RAET1H; RAET1L; ULBP2; ULBP-2/5/6; NKG2D ligand 2
Gene/Protein ID Q9BZM5
Species Human
Exact Sequence Gly26-Ser217
Molecular Weight The protein has a predicted MW of 24.6 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.
Activity Immobilized Biotinylated Human ULBP-2, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.2μg/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human ULBP-2, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.2μg/ml determined by ELISA.
Serial dilutions of Anti-NKG2D Antibody, hFc Tag were added into Biotinylated Human ULBP-2, His Tag : Human NKG2D, hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 45.2ng/ml.
The purity of Biotinylated Human ULBP-2 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human ULBP-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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