Product Description |
Recombinant Biotinylated Human ULBP-2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly26-Ser217. |
Product Background |
ULBP2 expression was however linked to expression of mature CD57() NK cells. In particular, expression of ULBP2 was strongest on those NK cells that had evidence of recent activation and proliferation. We suggest that ULBP2 could be used to identify recently activated "mature" NK cells. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Protein |
ULBP-2 |
Synonyms |
ALCAN-alpha; N2DL2; NKG2DL2; RAET1H; RAET1L; ULBP2; ULBP-2/5/6; NKG2D ligand 2 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Gly26-Ser217 |
Molecular Weight |
The protein has a predicted MW of 24.6 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human ULBP-2, His-Avi Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.30ug/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Immobilized Biotinylated Human ULBP-2, His-Avi Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.30ug/ml determined by ELISA (QC Test). |
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Biotinylated Human ULBP-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Serial dilutions of Anti-NKG2D Antibody, hFc Tag were added into Biotinylated Human ULBP-2, His Tag : Human NKG2D, hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 45.2ng/ml. |
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The purity of Biotinylated Human ULBP-2 is greater than 95% as determined by SEC-HPLC. |
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