Product Description |
Recombinant Cynomolgus AXL Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu33-Pro449. |
Product Background |
Axl, a member of the TAM (Tyro3, Axl, Mer) family, and its inhibitors can specifically break the kinase signaling nodes, allowing advanced patients to regain drug sensitivity with improved therapeutic efficacy. Overexpression and activation of Axl receptor tyrosine kinase have been widely accepted to promote cell proliferation, chemotherapy resistance, invasion, and metastasis in several human cancers, such as lung, breast, and pancreatic cancers. |
Product Category |
Recombinant Protein |
Target |
AXL |
Synonyms |
Axl; UFO; AXL oncogene; AXL; ARK; JTK11; Tyro7; AI323647; EC 2.7.10; EC 2.7.10.1 |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Glu33-Pro449 |
Molecular Weight |
The protein has a predicted MW of 46.3 kDa. Due to glycosylation, the protein migrates to 68-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus AXL, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-AXL Antibody, hFc Tag with the EC50 of 8.8ng/ml determined by ELISA (QC Test). The affinity constant of 1.70 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Cynomolgus Axl, His Tag immobilized on CM5 Chip can bind Cynomolgus GAS6, His Tag with an affinity constant of 1.70 nM as determined in SPR assay (Biacore T200). |
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Immobilized Cynomolgus AXL, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-AXL Antibody, hFc Tag with the EC50 of 8.8ng/ml determined by ELISA (QC Test). |
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Cynomolgus AXL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Cynomolgus AXL is greater than 95% as determined by SEC-HPLC. |
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