Cynomolgus AXL Protein (AXL-CM102)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Cynomolgus AXL, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-AXL Antibody, hFc Tag with the EC50 of 8.8ng/ml determined by ELISA (QC Test). The affinity constant of 1.70 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Glu33-Pro449

Catalog: AXL-CM102

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Product Description	Recombinant Cynomolgus AXL Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu33-Pro449.
Product Background	Axl, a member of the TAM (Tyro3, Axl, Mer) family, and its inhibitors can specifically break the kinase signaling nodes, allowing advanced patients to regain drug sensitivity with improved therapeutic efficacy. Overexpression and activation of Axl receptor tyrosine kinase have been widely accepted to promote cell proliferation, chemotherapy resistance, invasion, and metastasis in several human cancers, such as lung, breast, and pancreatic cancers.
Product Category	Recombinant Protein

Target	AXL
Synonyms	Axl; UFO; AXL oncogene; AXL; ARK; JTK11; Tyro7; AI323647; EC 2.7.10; EC 2.7.10.1
Gene/Protein ID	A0A1D5Q330
Species	Cynomolgus
Exact Sequence	Glu33-Pro449
Molecular Weight	The protein has a predicted MW of 46.3 kDa. Due to glycosylation, the protein migrates to 68-70 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Cynomolgus AXL, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-AXL Antibody, hFc Tag with the EC50 of 8.8ng/ml determined by ELISA (QC Test). The affinity constant of 1.70 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Cynomolgus Axl, His Tag immobilized on CM5 Chip can bind Cynomolgus GAS6, His Tag with an affinity constant of 1.70 nM as determined in SPR assay (Biacore T200).
	Immobilized Cynomolgus AXL, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-AXL Antibody, hFc Tag with the EC50 of 8.8ng/ml determined by ELISA (QC Test).
	The purity of Cynomolgus AXL is greater than 95% as determined by SEC-HPLC.
	Cynomolgus AXL on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.