Cynomolgus Coagulation factor III/Tissue Factor Protein (TFR-CM103)
Cynomolgus Coagulation factor III/Tissue Factor Protein (TFR-CM103)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus Coagulation Factor III, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Coagulation Factor III Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA.
  • Amino Acid Range: Gln34-Glu253
Catalog #TFR-CM103
Regular price $248.00
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Cynomolgus Coagulation factor III/Tissue Factor Protein (TFR-CM103) Summary

Product Description

Recombinant Cynomolgus Coagulation factor III/Tissue Factor Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gln34-Glu253.

Product Background

Tissue factor (also named Coagulation Factor III) is a cell surface glycoprotein responsible for initiating the extrinsic pathway of coagulation.Tissue factor is the primary cellular initiator of blood coagulation via interaction with coagulation factor VII. Aberrant expression of tissue factor is responsible for thrombosis during septic shock, atherosclerosis and cancer.

Product Category

Recombinant Protein / Antibody discovery / ADC targets

Cynomolgus Coagulation factor III/Tissue Factor Protein (TFR-CM103) Specifications/Details

Protein
Coagulation factor III/Tissue Factor
Synonyms
Tissue factor; Coagulation Factor III; TF; Thromboplastin; CD142; F3; FLJ17960; TFA
Accession
Species
Cynomolgus
Biotinylated
no
Amino Acid Range
Gln34-Glu253
Molecular Weight
The protein has a predicted MW of 26.65 kDa. Due to glycosylation, the protein migrates to 43-53 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Cynomolgus Coagulation Factor III, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Coagulation Factor III Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA.
Product Tag
C-His
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in 50mM Tris, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Cynomolgus Coagulation Factor III, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Coagulation Factor III Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA. Immobilized Cynomolgus Coagulation Factor III, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Coagulation Factor III Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA.
Cynomolgus Coagulation factor III on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Cynomolgus Coagulation factor III on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus Coagulation factor III is greater than 95% as determined by SEC-HPLC. The purity of Cynomolgus Coagulation factor III is greater than 95% as determined by SEC-HPLC.
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