- Express System: HEK293
- Product Tag: C-His
- Purity: > 95% as determined by Bis-Tris PAGE
- Activity: Immobilized Cynomolgus CRTAM, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CRTAM Antibody, hFc Tag with the EC50 of 22.6ng/ml determined by ELISA. See testing image for detail.
- Amino Acid Range: Ser18-Gly287
Product Description |
Recombinant Cynomolgus CRTAM Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser18-Gly287. |
Product Background |
Class-I Restricted T Cell-Associated Molecule (CRTAM) is a protein that is expressed after T cell activation. The interaction of CRTAM with its ligand, nectin-like 2 (Necl2), is required for the efficient production of IL-17, IL-22, and IFNγ by murine CD4 T cells, and it plays a role in optimal CD8 T and NK cell cytotoxicity. CRTAM promotes the pro-inflammatory cytokine profile; therefore, it may take part in the immunopathology of autoimmune diseases such as diabetes type 1 or colitis. |
Product Category |
Recombinant Protein |
Target |
CRTAM |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Ser18-Gly287 |
Molecular Weight |
The protein has a predicted MW of 30.80 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus CRTAM, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CRTAM Antibody, hFc Tag with the EC50 of 22.6ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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