Cynomolgus IL-18 R1/CD218a Protein (IL8-CM1R1)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 2.02 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Cys22-Gly329
Catalog #IL8-CM1R1
Regular price $315.00
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Product Description
Recombinant Cynomolgus IL-18 R1/CD218a Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Cys22-Gly329.
Product Background
Interleukin-18 (IL-18) is a member of the interleukin-1 family of cytokines produced constitutively by different cell types and by adipose tissue. Interleukin (IL)-18 was originally discovered as a factor that enhanced IFN-γ production from anti-CD3-stimulated Th1 cells, especially in the presence of IL-12. Upon stimulation with Ag plus IL-12, naïve T cells develop into IL-18 receptor (IL-18R) expressing Th1 cells, which increase IFN-γ production in response to IL-18 stimulation.
Product Category
Recombinant Protein
Target
IL-18 R1/CD218a
Accession
Species
Cynomolgus
Biotinylated
no
Amino Acid Range
Cys22-Gly329
Molecular Weight
The protein has a predicted MW of 36.45 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Activity
The affinity constant of 2.02 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Cynomolgus IL-18 R1, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-18, No Tag with an affinity constant of 2.02 nM as determined in SPR assay (Biacore T200).
Cynomolgus IL-18 R1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus IL-18 R1 is greater than 95% as determined by SEC-HPLC.

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