Cynomolgus IL-33 Protein (IL3-CM133)

Express System: HEK293
Product Tag: N-His
Purity: > 95% as determined by Tris-Bis PAGE
Activity: The affinity constant of 46.85 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: His109-Ile270

Catalog: IL3-CM133

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Product Description	Recombinant Cynomolgus IL-33 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains His109-Ile270.
Product Category	Recombinant Protein

Target	IL-33
Synonyms	C9orf26 Z; DVS27; IL1F11; NF-HEV; NFEHEV; IL33; DV27; C9ORF26; RP11-575C20.2
Gene/Protein ID	-A0A2K5W3I1-1
Species	Cynomolgus
Exact Sequence	His109-Ile270
Molecular Weight	The protein has a predicted MW of 19.2 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.
Activity	The affinity constant of 46.85 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human IL-1RL1, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-33, His Tag with an affinity constant of 46.85 nM as determined in SPR assay (Biacore T200).
	Cynomolgus IL-33 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.