Product Description |
Recombinant Cynomolgus IL-33 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains His109-Ile270. |
Product Background |
Interleukin-33 (IL-33) is a cytokine belonging to the IL-1 family, playing a role in inflammatory, infectious and autoimmune diseases and expressed in the cellular nucleus in several tissues. High levels of IL-33 are expressed in epithelial barrier tissues and endothelial barriers. ST2 is a receptor for IL-33, expressed selectively on a subset of Th2 cells, mediating some of their functions. |
Product Category |
Recombinant Protein |
Target |
IL-33 |
Synonyms |
C9orf26 Z; DVS27; IL1F11; NF-HEV; NFEHEV; IL33; DV27; C9ORF26; RP11-575C20.2 |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
His109-Ile270 |
Molecular Weight |
The protein has a predicted MW of 19.2 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus IL-33, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-33 Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA (QC Test). The affinity constant of 23.44 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
N-His |
Express System |
HEK293 |
Purity |
>95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human IL-1RL1, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus IL-33, His Tag with an affinity constant of 23.44 nM as determined in SPR assay (Biacore T200). |
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|
Immobilized Cynomolgus IL-33, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-33 Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA (QC Test). |
|
|
Cynomolgus IL-33 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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