Name: Cynomolgus IL-33 Protein (IL3-CM133)
Brand: KactusBio
SKU: IL3-CM133-100UG
Price: 379.5 USD
Availability: InStock

Express System: HEK293
Product Tag: N-His
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Cynomolgus IL-33, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-33 Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA (QC Test). The affinity constant of 23.44 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: His109-Ile270

Catalog #IL3-CM133

Size

100UG

500UG

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Product Description	Recombinant Cynomolgus IL-33 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains His109-Ile270.
Product Background	Interleukin-33 (IL-33) is a cytokine belonging to the IL-1 family, playing a role in inflammatory, infectious and autoimmune diseases and expressed in the cellular nucleus in several tissues. High levels of IL-33 are expressed in epithelial barrier tissues and endothelial barriers. ST2 is a receptor for IL-33, expressed selectively on a subset of Th2 cells, mediating some of their functions.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	IL-33
Synonyms	C9orf26 Z; DVS27; IL1F11; NF-HEV; NFEHEV; IL33; DV27; C9ORF26; RP11-575C20.2
Accession	A0A2K5W3I1-1
Species	Cynomolgus
Biotinylated	no
Amino Acid Range	His109-Ile270
Molecular Weight	The protein has a predicted MW of 19.2 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Cynomolgus IL-33, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-33 Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA (QC Test). The affinity constant of 23.44 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human IL-1RL1, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus IL-33, His Tag with an affinity constant of 23.44 nM as determined in SPR assay (Biacore T200).
	Immobilized Cynomolgus IL-33, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-33 Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA (QC Test).
	Cynomolgus IL-33 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Human IL-1RL1, hFc Tag on ProA-Biosensor, can bind Cynomolgus IL-33, His Tag with an affinity constant of 0.18 µM as determined in BLI assay (Gator).