Name: Human ACE2/ACEH Protein (ACE-HM101)
Brand: KactusBio
SKU: ACE-HM101-100UG
Price: 297.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human ACE2, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated SARS-COV-2 Spike RBD, hFc Tag with the EC50 of 27.2ng/ml determined by ELISA (QC Test). The affinity constant of 11.9nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Gln18-Ser740

Catalog #ACE-HM101

Size

100UG

500UG

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Product Description	Recombinant Human ACE2/ACEH Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln18-Ser740.
Product Background	ACE2 (Angiotensin I Converting Enzyme 2) is a Protein Coding gene. Diseases associated with ACE2 include Severe Acute Respiratory Syndrome and Neurogenic Hypertension.The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	ACE2/ACEH
Synonyms	ACE-2; ACEH; ACE2
Accession	Q9BYF1-1
Species	Human
Biotinylated	no
Amino Acid Range	Gln18-Ser740
Molecular Weight	The protein has a predicted MW of 84.7 kDa. Due to glycosylation, the protein migrates to 85-110 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human ACE2, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated SARS-COV-2 Spike RBD, hFc Tag with the EC50 of 27.2ng/ml determined by ELISA (QC Test). The affinity constant of 11.9nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	SARS-COV-2 Spike RBD captured on Protein A chip, can bind Human ACE2, His Tag with an affinity constant of 11.9nM as determined in a SPR assay (Biacore T200).
	Immobilized Human ACE2, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated SARS-COV-2 Spike RBD, hFc Tag with the EC50 of 27.2ng/ml determined by ELISA (QC Test).
	Human ACE2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human ACE2 is greater than 95% as determined by SEC-HPLC