Name: Human ANGPTL3/Angiopoietin-like 3 Protein (ANG-HM103)
Brand: KactusBio
SKU: ANG-HM103-100UG
Price: 223.3 USD
Availability: InStock

Human ANGPTL3/Angiopoietin-like 3 Protein (ANG-HM103)

Human ANGPTL3, His Tag immobilized on CM5 Chip can bind Anti-ANGPTL3 Antibody, hFc Tag with an affinity constant of 0.56 nM as determined in SPR assay (Biacore T200).

Immobilized Human ANGPTL3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-ANGPTL3 Antibody, hFc Tag with the EC50 of 16.0ng/ml determined by ELISA (QC Test).

Human ANGPTL3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human ANGPTL3 is greater than 90% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Human ANGPTL3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-ANGPTL3 Antibody, hFc Tag with the EC50 of 16.0ng/ml determined by ELISA (QC Test). The affinity constant of 0.56 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Ser17-Glu460

Catalog #ANG-HM103

Size

100UG

500UG

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Product Description	Recombinant Human ANGPTL3/Angiopoietin-like 3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser17-Glu460.
Product Background	ANGPTL3 is a secreted glycoprotein that is structurally related to the angiopoietins. Mature human ANGPTL3 contains an N-terminal coiled coil domain and a C‑terminal fibrinogen-like domain. ANGPTL3 is expressed in the liver from early in development through adulthood. Acts in part as a hepatokine that is involved in regulation of lipid and glucose metabolism. Proposed to play a role in the trafficking of energy substrates to either storage or oxidative tissues in response to food intake.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	ANGPTL3/Angiopoietin-like 3
Synonyms	ANGPTL3; ANGPT5; ANG-5; Angiopoietin-5; FHBL2; ANL3; Angiopoietin-5; Angiopoietin-like Protein 3
Accession	Q9Y5C1
Species	Human
Biotinylated	no
Amino Acid Range	Ser17-Glu460
Molecular Weight	The protein has a predicted MW of 52.90 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human ANGPTL3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-ANGPTL3 Antibody, hFc Tag with the EC50 of 16.0ng/ml determined by ELISA (QC Test). The affinity constant of 0.56 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human ANGPTL3, His Tag immobilized on CM5 Chip can bind Anti-ANGPTL3 Antibody, hFc Tag with an affinity constant of 0.56 nM as determined in SPR assay (Biacore T200).
	Immobilized Human ANGPTL3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-ANGPTL3 Antibody, hFc Tag with the EC50 of 16.0ng/ml determined by ELISA (QC Test).
	Human ANGPTL3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human ANGPTL3 is greater than 90% as determined by SEC-HPLC.

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