Human B7-H3 (4Ig) /B7-H3b Protein (BH7-HM23B)
Catalog: BH7-HM23B
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Product Description | Recombinant Human B7-H3 (4Ig)/B7-H3b Protein is expressed from Expi293 with hFc tag at the C-terminal.It contains Gly27-Thr461. |
Product Category | Recombinant Protein |
Target | B7-H3 (4Ig)/B7-H3b |
Synonyms | B7-H3; CD276; PSEC0249; 4Ig-B7-H3; UNQ309; PRO352; B7 homolog 3; CD276 |
Gene/Protein ID | Q5ZPR3-1 |
Species | Human |
Exact Sequence | Gly27-Thr461 |
Molecular Weight | The protein has a predicted MW of 73.4 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Tris-Bis PAGE result. |
Activity | Immobilized Human B7-H3 (4Ig) , hFc Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-B7-H3 Antibody, hFc Tag with the EC50 of 44.3ng/ml determined by ELISA. See testing image for detail. |
Product Tag | C-hFc |
Express System | Expi293 |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 1EU per μg by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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