Name: Non-biotinylated Human BCMA/TNFRSF17 Protein (BCM-HM117)
Brand: KactusBio
SKU: BCM-HM117-100UG
Price: 330.0 USD
Availability: InStock

Non-biotinylated Human BCMA/TNFRSF17 Protein (BCM-HM117)

Immobilized Human BCMA, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human BAFF, His Tag with the EC50 of 10.1 ng/ml determined by ELISA (QC Test).

Immobilized Human BCMA, His Tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-BCMA Antibody, hFc Tag with the EC50 of 2.4 ng/ml determined by ELISA.

Human BCMA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Loaded Biotinylated Human BAFF, His-Avi Tag on Streptavidin-Biosensor can bind Human BCMA, His Tag with an affinity constant of 0.65 µM as determined in BLI assay (Gator® Prime).

Loaded Anti-BCMA Antibody on ProA-Biosensor can bind Human BCMA, His Tag with an affinity constant of 16.10 nM as determined in BLI assay (Gator® Prime).

The purity of Human BCMA is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human BCMA, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human BAFF, His Tag with the EC50 of 10.1 ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Met1-Ala54

Catalog #BCM-HM117

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Non-biotinylated Human BCMA/TNFRSF17 Protein (BCM-HM117) Summary

Product Description	Recombinant Human BCMA/TNFRSF17 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Met1-Ala54.
Product Background	B-cell maturation antigen (BCMA or BCM), also known as tumor necrosis factor receptor superfamily member 17 (TNFRSF17), is a protein that in humans is encoded by the TNFRSF17 gene. TNFRSF17 is a cell surface receptor of the TNF receptor superfamily which recognizes B-cell activating factor (BAFF).
Product Category	Recombinant Protein / Antibody discovery / CAR-T targets

Non-biotinylated Human BCMA/TNFRSF17 Protein (BCM-HM117) Specifications/Details

Protein	BCMA/TNFRSF17
Synonyms	CD269; TNFRSF17; BCMA; BCM; TNFRSF13A
Accession	Q02223-1
Species	Human
Biotinylated	no
Amino Acid Range	Met1-Ala54
Molecular Weight	The protein has a predicted MW of 8.9 kDa. Due to glycosylation, the protein migrates to 15-25 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human BCMA, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human BAFF, His Tag with the EC50 of 10.1 ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human BCMA, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human BAFF, His Tag with the EC50 of 10.1 ng/ml determined by ELISA (QC Test).
	Immobilized Human BCMA, His Tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-BCMA Antibody, hFc Tag with the EC50 of 2.4 ng/ml determined by ELISA.
	Human BCMA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Biotinylated Human BAFF, His-Avi Tag on Streptavidin-Biosensor can bind Human BCMA, His Tag with an affinity constant of 0.65 µM as determined in BLI assay (Gator® Prime).
	Loaded Anti-BCMA Antibody on ProA-Biosensor can bind Human BCMA, His Tag with an affinity constant of 16.10 nM as determined in BLI assay (Gator® Prime).
	The purity of Human BCMA is greater than 95% as determined by SEC-HPLC.