Human BTN2A1 Protein (BTN-HM12A)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 0.12 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Gln29-Ala248

Catalog: BTN-HM12A

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Product Description Recombinant Human BTN2A1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln29-Ala248.

Product Background

The MHC-encoded butyrophilin, BTN2A1, is a cell surface glycoprotein related to the extended family of B7 costimulatory molecules. BTN2A1 mRNA was expressed in most human tissues, but protein expression was significantly lower in leukocytes.

Product Category

Recombinant Protein

Target

BTN2A1

Gene/Protein ID

Q7KYR7-1

Species

Human

Exact Sequence

Gln29-Ala248
Molecular Weight The protein has a predicted MW of 25.65 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Tris-Bis PAGE result.

Activity

The affinity constant of 0.12 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human BTN2A1, His Tag immobilized on CM5 Chip can bind Human CD209, His Tag with an affinity constant of 0.12 uM as determined in SPR assay (Biacore T200).
The purity of Human BTN2A1 is greater than 95% as determined by SEC-HPLC.
Human BTN2A1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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