Human CD117 Protein (CD1-HM217)
Human CD117 Protein (CD1-HM217)
Human CD117 Protein (CD1-HM217)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human SCF at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CD117, hFc Tag with the EC50 of 11.3ng/ml determined by ELISA (QC Test).
  • Amino Acid Range: Gln26-Pro520
Catalog #CD1-HM217
Regular price $280.50
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Human CD117 Protein (CD1-HM217) Summary

Product Description

Recombinant Human CD117 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln26-Pro520.

Product Background

The c-kit proto-oncogen (CD 117) has been shown to be present in several cell types including normal and neoplastic hemopoietic cells. Among normal BM cells, CD117 expression has been found in about half of the CD34 precursors including progenitors committed to the erythroid, granulo-monocytic, and megakaryocytic cell lineages.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human CD117 Protein (CD1-HM217) Specifications/Details

Protein
CD117
Synonyms
SCFR; PBT; CD117; ckit; c-kit; EC 2.7.10; EC 2.7.10.1; KIT
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Gln26-Pro520
Molecular Weight
The protein has a predicted MW of 82.46 kDa. Due to glycosylation, the protein migrates to 100-130 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human SCF at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CD117, hFc Tag with the EC50 of 11.3ng/ml determined by ELISA (QC Test).
Product Tag
C-hFc
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human SCF at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CD117, hFc Tag with the EC50 of 11.3ng/ml determined by ELISA (QC Test). Immobilized Human SCF at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CD117, hFc Tag with the EC50 of 11.3ng/ml determined by ELISA (QC Test).
Immobilized Human CD117, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-CD117 Antibody, hFc Tag with the EC50 of 22.7ng/ml determined by ELISA. Immobilized Human CD117, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-CD117 Antibody, hFc Tag with the EC50 of 22.7ng/ml determined by ELISA.
Human CD117 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human CD117 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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