Product Description |
Recombinant Human CD13/ANPEP Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Lys69-Lys967. |
Product Background |
CD13/aminopeptidase N is a widely expressed ectoenzyme with multiple functions. As an enzyme, CD13 regulates activities of numerous cytokines by cleaving their N-terminals and is involved in Ag processing by trimming the peptides bound to MHC class II. Independent of its enzymatic activity, cell membrane CD13 functions by cross-linking-induced signal transduction, regulation of receptor recycling, enhancement of FcγR-mediated phagocytosis, and acting as a receptor for cytokines. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Protein |
CD13/ANPEP |
Synonyms |
AP-N; AP-M; ANPEP; APN; CD13; PEPN; Cd13; CD13APN; gp150; LAP1; PEPNhAPN |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Lys69-Lys967 |
Molecular Weight |
The protein has a predicted MW of 103.8 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Human CD13 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human CD13 is greater than 95% as determined by SEC-HPLC. |
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