Product Description |
Recombinant Human CD300LF Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Thr20-Ser156. |
Product Background |
Murine norovirus (MNoV) is an important model of human norovirus (HNoV) and mucosal virus infection more broadly. CD300lf as a proteinaceous receptor for MNoV. Interestingly, its paralogue CD300ld was also sufficient for MNoV infection in vitro. CD300lf is essential for both oral and parenteral MNoV infection and to elicit anti-MNoV humoral responses in vivo. human CD300lf (huCD300lf) is not essential for HNoV infection, nor does huCD300lf inhibit binding of HNoV virus-like particles to glycans. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Protein |
CD300LF |
Synonyms |
CLM-1; IREM-1; IgSF13; CD300F; IGSF13; NKIR; CD300f; CD300LF; CLIM1; DIgR2; LMIR3; NKIR; Pigr3 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Thr20-Ser156 |
Molecular Weight |
The protein has a predicted MW of 42.1 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-hFc |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Human CD300LF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human CD300LF is greater than 95% as determined by SEC-HPLC. |
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