Name: Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein (CD3-HM157)
Brand: KactusBio
SKU: CD3-HM157-100UG
Price: 412.5 USD
Availability: InStock

Sensorgram plot from SPR analysis showing binding kinetics of multiple analyte concentrations to immobilized ligand over time.

Human CD3E&CD3G, His Tag captured on CM5 Chip via Anti-His Antibody can bind OKT3, mFc Tag with an affinity constant of 0.22 nM as determined in SPR assay (Biacore T200).

Immobilized Human CD3E&CD3G, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 110.2 ng/ml determined by ELISA (QC Test).

Immobilized Human CD3E&CD3G, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Foralumab, hFc Tag with the EC50 of 36.2 ng/ml determined by ELISA.

Human CD3E&CD3G on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human CD3E&CD3G is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CD3E&CD3G, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 110.2 ng/ml determined by ELISA (QC Test). The affinity constant of 0.22 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Asp23-Asp126(CD3E) & Gln23-Ser116(CD3G)

Catalog #CD3-HM157

Size

100UG

500UG

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Product Description	Recombinant Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein is expressed from HEK293 with His tag at the C-Terminus of CD3G.It contains Asp23-Asp126 (CD3E) and Gln23-Ser116 (CD3G).
Product Background	T-cell surface glycoprotein CD3 epsilon&CD3 gamma chain, also known as CD3E & CD3G, are single-pass type I membrane proteins. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	CD3E&CD3G/CD3 epsilon&CD3 gamma
Synonyms	CD3; CD3 epsilon; CD3epsilon; CD3ε; CD3 ε; CD3E; CD3e; CD3 gamma; CD3gamma; CD3γ; CD3 γ; CD3G; CD3g; CD3epsilon&CD3gamma; CD3 epsilon&CD3 gamma; CD3 epsilon & CD3 gamma; CD3ε&CD3γ; CD3 ε&CD3 γ; CD3 ε & CD3 γ; CD3E&CD3G; CD3E & CD3G; CD3 E & CD3 G; CD3e&CD3g; CD3e & CD3g; CD3 e & CD3 g
Accession	P07766(CD3E)&P09693(CD3G)
Species	Human
Biotinylated	no
Amino Acid Range	Asp23-Asp126(CD3E) & Gln23-Ser116(CD3G)
Molecular Weight	The protein has a predicted MW of 17.9 kDa (CD3E) and 17.9 kDa (CD3G). Due to glycosylation, the protein migrates to 25-40 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human CD3E&CD3G, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 110.2 ng/ml determined by ELISA (QC Test). The affinity constant of 0.22 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human CD3E&CD3G, His Tag captured on CM5 Chip via Anti-His Antibody can bind OKT3, mFc Tag with an affinity constant of 0.22 nM as determined in SPR assay (Biacore T200).
	Immobilized Human CD3E&CD3G, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 110.2 ng/ml determined by ELISA (QC Test).
	Immobilized Human CD3E&CD3G, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Foralumab, hFc Tag with the EC50 of 36.2 ng/ml determined by ELISA.
	Human CD3E&CD3G on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human CD3E&CD3G is greater than 95% as determined by SEC-HPLC.

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