Product Description |
Recombinant Human CD45/PTPRC Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln26-Lys577. |
Product Background |
PTPRC (also known as CD45),T cells require the protein tyrosine phosphatase CD45 to detect and respond to antigen because it activates the Src family kinase Lck, which phosphorylates the T cell antigen receptor (TCR) complex. CD45 ativates Lck by opposing the negative regulatory kinase Csk. Paradoxically, CD45 has also been implicated in suppressing TCR signaling by dephosphorylating the same signaling motifs within the TCR complex upon which Lck acts. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Protein |
CD45/PTPRC |
Synonyms |
L-CA; CD45; T200; PTPRC; B220; CD45R; GP180; LCA; LY5; EC 3.1.3.48 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln26-Lys577 |
Molecular Weight |
The protein has a predicted MW of 87.53 kDa. Due to glycosylation, the protein migrates to 120-160 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized AHuman CD45, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-CD45 Antibody, hFc Tag with the EC50 of 0.16ug/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-hFc |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Immobilized AHuman CD45, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-CD45 Antibody, hFc Tag with the EC50 of 0.16ug/ml determined by ELISA. |
|
|
Human CD45 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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