ELISA binding curve showing dose-dependent interaction between human CD83 protein and anti-CD83 antibody.
Human CD83 Protein (CD8-HM183)
Human CD83 Protein (CD8-HM183)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human CD83, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD83 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Thr20-Ala143
Catalog #CD8-HM183
Regular price $223.30
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Product Description

Recombinant Human CD83 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr20-Ala143.

Product Background

CD83 is a member of the immunoglobulin (Ig) superfamily and is expressed in membrane bound or soluble forms. Membrane CD83 (mCD83) can be detected on a variety of activated immune cells, although it is most highly and stably expressed by mature dendritic cells (DC). While CD83 is emerging as a promising immune modulator with therapeutic potential, some important aspects such as its ligand/s, intracellular signaling pathways and modulators of its expression are unclear.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others
Protein
CD83
Synonyms
CD83; HB15; BL11; CD83 molecule
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Thr20-Ala143
Molecular Weight
The protein has a predicted MW of 15.2 kDa. Due to glycosylation, the protein migrates to 18 kDa and 25-28 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human CD83, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD83 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human CD83, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD83 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test). Immobilized Human CD83, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD83 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test).
Human CD83 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human CD83 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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