Product Description |
Recombinant Human CEACAM-3/CD66d Protein is expressed from HEK293 with hFc tag at the C-terminus.It contains Lys35-Gly155. |
Product Background |
The human granulocyte-specific receptor carcinoembryonic antigen-related cell adhesion molecule (CEACAM)3 is critically involved in the opsonin-independent recognition of several bacterial pathogens. CEACAM3-mediated phagocytosis depends on the integrity of an ITAM-like sequence within the cytoplasmic domain of CEACAM3 and is characterized by rapid stimulation of the GTPase Rac. |
Product Category |
Recombinant Protein / Antibody discovery / CAR-T targets |
Protein |
CEACAM-3/CD66d |
Synonyms |
CEACAM3; CD66d; CGM1; CEA; |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Lys35-Gly155 |
Molecular Weight |
The protein has a predicted MW of 39.85 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human CEACAM-7, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CEACAM-3, hFc Tag with the EC50 of 31.1ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-hFc |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Immobilized Human CEACAM-7, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CEACAM-3, hFc Tag with the EC50 of 31.1ng/ml determined by ELISA. |
|
|
Human CEACAM-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human CEACAM-3 is greater than 95% as determined by SEC-HPLC. |
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