Human Complement Factor D/CFD Protein (CFD-HM101)
Human Complement Factor D/CFD Protein (CFD-HM101)
Human Complement Factor D/CFD Protein (CFD-HM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Complement Factor D, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Complement Factor D Antibody, hFc Tag with the EC50 of 18.1ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Ile26-Ala253
Catalog #CFD-HM101
Regular price $346.50
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Product Description

Recombinant Human Complement Factor D/CFD Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile26-Ala253.

Product Background

Complement factor D is a serine protease essential for the activation of the alternative pathway and is expressed in the kidney, adipocytes, and macrophages. Factor D is found at relatively high levels in glomeruli suggesting that this component of the complement cascade could influence renal pathophysiology.Complement factor D or alternative pathway activation is needed to prevent spontaneous accumulation of C3 and IgM deposits within the mesangium.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others
Protein
Complement Factor D/CFD
Synonyms
Adipsin; C3 convertase activator; Complement factor D; CFD; PFD; DF; ADN; FACTOR D; AMBP-1; EC 3.4.21; EC 3.4.21.46
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Ile26-Ala253
Molecular Weight
The protein has a predicted MW of 25.5 kDa. Due to glycosylation, the protein migrates to 26-27 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human Complement Factor D, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Complement Factor D Antibody, hFc Tag with the EC50 of 18.1ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human Complement Factor D, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Complement Factor D Antibody, hFc Tag with the EC50 of 18.1ng/ml determined by ELISA (QC Test). Immobilized Human Complement Factor D, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Complement Factor D Antibody, hFc Tag with the EC50 of 18.1ng/ml determined by ELISA (QC Test).
Human Complement Factor D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human Complement Factor D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Complement Factor D is greater than 95% as determined by SEC-HPLC. The purity of Human Complement Factor D is greater than 95% as determined by SEC-HPLC.
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