Human DKK1 C terminal Domain Protein (DKK-HM51C)

Immobilized Human DKK1 C terminal Domain, hFc Tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for Biotinylated Anti-DKK1 Antibody, hFc Tag with the EC50 of 26.7 ng/ml determined by ELISA.

Human DKK1 C terminal Domain on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Express System: HEK293
Product Tag: C-hFc-Avi
Purity: >95% as determined by Bis-Tris PAGE
Activity: Immobilized Human DKK1 C terminal Domain, hFc Tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for Biotinylated Anti-DKK1 Antibody, hFc Tag with the EC50 of 26.7 ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Met178-His266

Catalog #DKK-HM51C

Size

100UG

500UG

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Product Description	Recombinant Human DKK1 C terminal Domain Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus.It contains Met178-His266.
Product Background	Dickkopf-1 (Dkk1), the founding and best-studied member of the Dkk family, functions as an antagonist of canonical Wnt/β-catenin. Dkk1 is considered to play a broad role in a variety of biological processes.
Product Category	Recombinant Protein / Antibody discovery

Protein	DKK1 C terminal Domain
Synonyms	Dickkopf-related 1; Dickkopf-1; Dkk-1; Dkk1; hDkk-1; hDkk1; SK
Accession	O94907
Species	Human
Biotinylated	no
Amino Acid Range	Met178-His266
Molecular Weight	The protein has a predicted MW of 38.69 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human DKK1 C terminal Domain, hFc Tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for Biotinylated Anti-DKK1 Antibody, hFc Tag with the EC50 of 26.7 ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-hFc-Avi
Expression System	HEK293
Purity	>95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 20mM NaAc,150mM NaCl (pH 5.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 20mM NaAc,150mM NaCl (pH 5.0).
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human DKK1 C terminal Domain, hFc Tag at 0.5 ug/ml (100 ul/Well) on the plate. Dose response curve for Biotinylated Anti-DKK1 Antibody, hFc Tag with the EC50 of 26.7 ng/ml determined by ELISA.
	Human DKK1 C terminal Domain on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.