Human E-Selectin/CD62E Protein (ESE-HM201)
Human E-Selectin/CD62E Protein (ESE-HM201)
Human E-Selectin/CD62E Protein (ESE-HM201)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human E-Selectin, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-E-Selectin Antibody, hFc Tag with the EC50 31.4ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Trp22-Pro556
Catalog #ESE-HM201
Regular price $198.00
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Product Description

Recombinant Human E-Selectin/CD62E Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Trp22-Pro556.

Product Background

E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 - 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha.E-selectin mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others
Protein
E-Selectin/CD62E
Synonyms
CD62E; ELAM; ELAM1; ESEL; E-Selectin; LECAM2; SELE; selectin E; RP1-117P20.2
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Trp22-Pro556
Molecular Weight
The protein has a predicted MW of 85.4 kDa. Due to glycosylation, the protein migrates to 90-130 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human E-Selectin, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-E-Selectin Antibody, hFc Tag with the EC50 31.4ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-hFc
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human E-Selectin, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-E-Selectin Antibody, hFc Tag with the EC50 31.4ng/ml determined by ELISA (QC Test). Immobilized Human E-Selectin, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-E-Selectin Antibody, hFc Tag with the EC50 31.4ng/ml determined by ELISA (QC Test).
Human E-selectin on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. Human E-selectin on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Human E-selectin is greater than 95% as determined by SEC-HPLC. The purity of Human E-selectin is greater than 95% as determined by SEC-HPLC.
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