Product Description |
Recombinant Human EGFR/HER1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.; It contains Leu25-Ser645. |
Product Background |
The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. |
Product Category |
Recombinant Protein / Antibody discovery / CAR-T targets |
Protein |
EGFR/HER1 |
Synonyms |
ErbB; EC 2.7.10; EC 2.7.10.1; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Leu25-Ser645 |
Molecular Weight |
The protein has a predicted MW of 95.2 kDa. Due to glycosylation, the protein migrates to 110-140 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human EGF, No Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 0.44 ug/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-hFc |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Immobilized Human EGF, No Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 0.44 ug/ml determined by ELISA (QC Test). |
|
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Human EGFR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of-Human EGFR is greater than 95% as determined by SEC-HPLC. |
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