Human Ficolin 1/FCN1 Protein (FCN-HM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Amino Acid Range: Ala30-Ala326
Catalog #FCN-HM101
Regular price $297.00
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Product Description

Recombinant Human ficolin 1/FCN1 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ala30-Ala326.

Product Background

The ficolins are members of a recently discovered family of host innate opsonins that can activate the lectin pathway of complement. The ficolins bind many ligands, although they are typically described as binding acetylated sugars. Ficolin-1 is the least-described of the Ficolins and is expressed by monocytes, granulocytes, and in the lungs. Ficolin-1 is found circulating at low concentrations in serum but is regarded primarily as a secretory molecule that exerts its function locally in inflamed tissues.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others
Protein
Ficolin 1/FCN1
Synonyms
ficolin 1; FCN1; ficolin1; Ficolin-A; M-ficolin; FCNM
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Ala30-Ala326
Molecular Weight
The protein has a predicted MW of 33.11 kDa. Due to glycosylation, the protein migrates to 34-38 kDa based on Bis-Tris PAGE result.

Product Tag

C-His
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS, 350mM NaCl, 200mM Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human ficolin 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human ficolin 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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