Name: Human G-CSF Protein (CSF-HM201)
Brand: KactusBio
SKU: CSF-HM201-100UG
Price: 297.0 USD
Availability: InStock

Express System: HEK293
Product Tag: N-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human G-CSF R, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human G-CSF, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Thr31-Pro204

Catalog #CSF-HM201

Size

100UG

500UG

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Product Description	Recombinant Human G-CSF Protein is expressed from HEK293 with hFc tag at the N-terminus.It contains Thr31-Pro204.
Product Background	Granulocyte colony-stimulating factor (G-CSF) is an hemopoietic growth factor produced by fibroblasts, monocytes and endothelial cells. G-CSF is expressed by numerous cell types including neuronal, immune and endothelial cells. G-CSF is released in autocrine manner and binds to its receptor G-CSF-R which further activates numerous signaling transduction pathways including PI3K/AKT, JAK/STAT and MAP kinase, and thereby promote neuronal survival, proliferation, differentiation, mobilization of hematopoietic stem and progenitor cells.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	G-CSF
Synonyms	CSF3; GCSF; C17orf33; CSF3OS;
Accession	NP_757373.1
Species	Human
Biotinylated	no
Amino Acid Range	Thr31-Pro204
Molecular Weight	The protein has a predicted MW of 44.20 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human G-CSF R, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human G-CSF, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	N-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human G-CSF R, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human G-CSF, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA (QC Test).
	Human G-CSF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human G-CSF is greater than 95% as determined by SEC-HPLC.