Name: Human Galectin 3 Protein (GLT-HM103)
Brand: KactusBio
SKU: GLT-HM103-100UG
Price: 264.0 USD
Availability: InStock

Express System: HEK293
Product Tag: N-His
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Human Galectin 3, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-Galectin 3 Antibody, hFc Tag with the EC50 of 6.8 ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Ala2-Ile250

Catalog #GLT-HM103

Size

100UG

500UG

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Product Description	Recombinant Human Galectin 3 Protein is expressed from HEK293 with His tag at the N-Terminus.; It contains Ala2-Ile250.
Product Background	Human Galectin-3, also known as Mac-2, L29, CBP35, and epsilon BP, is classified as a chimeric member of the Galectin superfamily and contains one carbohydrate recognition domain (CRD) linked to a nonlectin domain.Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity).
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	Galectin 3
Synonyms	AGE-R3; CBP35; GAL3; Gal-3; galactin-3; GALBPCBP35; Galectin3; GALIG; L29; L31; Lectin L-29; LGALS2; LGALS3; Mac-2; MAC2GAL3
Accession	P17931
Species	Human
Biotinylated	no
Amino Acid Range	Ala2-Ile250
Molecular Weight	The protein has a predicted MW of 27.1 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human Galectin 3, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-Galectin 3 Antibody, hFc Tag with the EC50 of 6.8 ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	N-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 50 mM HEPES, 150 mM NaCl, 5 mM EDTA, 2 mM TCEP (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human Galectin 3, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-Galectin 3 Antibody, hFc Tag with the EC50 of 6.8 ng/ml determined by ELISA (QC Test).
	Human Galectin 3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Anti-Galectin 3 Ab., hFc-Avi Tag on ProA-Biosensor can bind Human Galectin 3, His Tag with an affinity constant of 60.90 nM as determined in BLI assay (Gator® Prime).