Name: Human GDNF Protein (GDF-HE001)
Brand: KactusBio
SKU: GDF-HE001-100UG
Price: 379.5 USD
Availability: InStock

Express System: E.coli
Product Tag: No Tag
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human GDNF, No Tag at 1 ug/ml (100ul/well) on the plate. Dose response curve for Human GFR alpha 1, His Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Ser78-Ile211

Catalog #GDF-HE001

Size

100UG

500UG

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Product Description	Recombinant Human GDNF Protein is expressed from E.coli without tagIt contains Ser78-Ile211.
Product Background	Glial cell line-derived neurotrophic factor (GDNF) is a 134 amino acid protein belonging in the GDNF family ligands (GFLs). GDNF was originally isolated from rat glial cell lines and identified as a neurotrophic factor with the ability to promote dopamine uptake within midbrain dopaminergic neurons.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	GDNF
Synonyms	Glial cell line-derived neurotrophic factor; hGDNF; Astrocyte-derived trophic factor (ATF)
Accession	P39905-1
Species	Human
Biotinylated	no
Amino Acid Range	Ser78-Ile211
Molecular Weight	The protein has a predicted MW of 15.06 kDa same as Bis-Tris PAGE result.
Activity	Immobilized Human GDNF, No Tag at 1 ug/ml (100ul/well) on the plate. Dose response curve for Human GFR alpha 1, His Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	No Tag
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human GDNF, No Tag at 1 ug/ml (100ul/well) on the plate. Dose response curve for Human GFR alpha 1, His Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test).
	Human GDNF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells. The ED50-for this effect is 2‑12 ng/mL in the presence of Recombinant Human GFR alpha ‑1/GDNF-R alpha ‑1 Fc-Chimera.
	The purity of Human GDNF is greater than 95% as determined by SEC-HPLC.