Name: Human GITR Ligand/TNFSF18 Trimer Protein (FSF-HM218)
Brand: KactusBio
SKU: FSF-HM218-100UG
Price: 315.0 USD
Availability: InStock

Human GITR Ligand/TNFSF18 Trimer Protein (FSF-HM218)

Human GITR, His Tag captured on CM5 Chip via Anti-his antibody can bind Human GITR Ligand Trimer, hFc Tag with an affinity constant of 4.47 nM as determined in SPR assay (Biacore T200).

Immobilized Human GITR Ligand Trimer, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human GITR, His Tag with the EC50 of 2.60ug/ml determined by ELISA (QC Test).

Human GITR Ligand (Trimer) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human GITR Ligand (Trimer) is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: N-monomeric hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human GITR Ligand Trimer, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human GITR, His Tag with the EC50 of 2.60ug/ml determined by ELISA (QC Test). The affinity constant of 4.47 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Gln50-Ser177

Catalog #FSF-HM218

Size

100UG

500UG

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Product Description	Recombinant Human GITR Ligand/TNFSF18 Trimer Protein is expressed from HEK293 with monomeric hFc tag at the N-Terminus.It contains Gln50-Ser177.
Product Background	Glucocorticoid-induced TNFR-related protein (TNFRSF18, GITR, CD357), expressed by T cells, and its ligand (TNFSF18, GITRL), expressed by myeloid populations, provide co-stimulatory signals that boost T cell activity. Due to the important role that GITR plays in regulating immune functions, agonistic stimulation of GITR is a promising therapeutic concept.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein	GITR Ligand/TNFSF18 Trimer
Synonyms	TNFSF18; AITRL; TL6; GITRL; GITR Ligand
Accession	Q9UNG2
Species	Human
Biotinylated	no
Amino Acid Range	Gln50-Ser177
Molecular Weight	The protein has a predicted MW of 70.2 kDa. Due to glycosylation, the protein migrates to 75-85 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human GITR Ligand Trimer, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human GITR, His Tag with the EC50 of 2.60ug/ml determined by ELISA (QC Test). The affinity constant of 4.47 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-monomeric hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human GITR, His Tag captured on CM5 Chip via Anti-his antibody can bind Human GITR Ligand Trimer, hFc Tag with an affinity constant of 4.47 nM as determined in SPR assay (Biacore T200).
	Immobilized Human GITR Ligand Trimer, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human GITR, His Tag with the EC50 of 2.60ug/ml determined by ELISA (QC Test).
	Human GITR Ligand (Trimer) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human GITR Ligand (Trimer) is greater than 95% as determined by SEC-HPLC.