Human GITR Ligand/TNFSF18 Trimer Protein (FSF-HM218)
Human GITR Ligand/TNFSF18 Trimer Protein (FSF-HM218)
Human GITR Ligand/TNFSF18 Trimer Protein (FSF-HM218)
Human GITR Ligand/TNFSF18 Trimer Protein (FSF-HM218)
  • Express System: HEK293
  • Product Tag: N-monomeric hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human GITR Ligand Trimer, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human GITR, His Tag with the EC50 of 2.60ug/ml determined by ELISA (QC Test). The affinity constant of 4.47 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gln50-Ser177
Catalog #FSF-HM218
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Product Description
 Recombinant Human GITR Ligand/TNFSF18 Trimer Protein is expressed from HEK293 with monomeric hFc tag at the N-Terminus.It contains Gln50-Ser177.
Product Background
 Glucocorticoid-induced TNFR-related protein (TNFRSF18, GITR, CD357), expressed by T cells, and its ligand (TNFSF18, GITRL), expressed by myeloid populations, provide co-stimulatory signals that boost T cell activity. Due to the important role that GITR plays in regulating immune functions, agonistic stimulation of GITR is a promising therapeutic concept.
Product Category
 Recombinant Protein
Target
 GITR Ligand/TNFSF18 Trimer
Synonyms
 TNFSF18; AITRL; TL6; GITRL; GITR Ligand
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gln50-Ser177
Molecular Weight
 The protein has a predicted MW of 70.2 kDa. Due to glycosylation, the protein migrates to 75-85 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human GITR Ligand Trimer, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human GITR, His Tag with the EC50 of 2.60ug/ml determined by ELISA (QC Test). The affinity constant of 4.47 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 N-monomeric hFc
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human GITR, His Tag captured on CM5 Chip via Anti-his antibody can bind Human GITR Ligand Trimer, hFc Tag with an affinity constant of 4.47 nM as determined in SPR assay (Biacore T200).
Immobilized Human GITR Ligand Trimer, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human GITR, His Tag with the EC50 of 2.60ug/ml determined by ELISA (QC Test).
Human GITR Ligand (Trimer) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human GITR Ligand (Trimer) is greater than 95% as determined by SEC-HPLC.
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