Human GITR Ligand/TNFSF18 Trimer Protein (FSF-HM218)

  • Express System: HEK293
  • Product Tag: N-monomeric hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human GITR Ligand Trimer, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human GITR, His Tag with the EC50 of 2.60ug/ml determined by ELISA (QC Test). The affinity constant of 4.47 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gln50-Ser177

Catalog: FSF-HM218

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Product Description Recombinant Human GITR Ligand/TNFSF18 Trimer Protein is expressed from HEK293 with monomeric hFc tag at the N-Terminus.It contains Gln50-Ser177.

Product Background

Glucocorticoid-induced TNFR-related protein (TNFRSF18, GITR, CD357), expressed by T cells, and its ligand (TNFSF18, GITRL), expressed by myeloid populations, provide co-stimulatory signals that boost T cell activity. Due to the important role that GITR plays in regulating immune functions, agonistic stimulation of GITR is a promising therapeutic concept.

Product Category

Recombinant Protein

Target

GITR Ligand/TNFSF18 Trimer
Synonyms TNFSF18; AITRL; TL6; GITRL; GITR Ligand
Accession

Species

Human

Amino Acid Range

Gln50-Ser177
Molecular Weight The protein has a predicted MW of 70.2 kDa. Due to glycosylation, the protein migrates to 75-85 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human GITR Ligand Trimer, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human GITR, His Tag with the EC50 of 2.60ug/ml determined by ELISA (QC Test). The affinity constant of 4.47 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

N-monomeric hFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human GITR, His Tag captured on CM5 Chip via Anti-his antibody can bind Human GITR Ligand Trimer, hFc Tag with an affinity constant of 4.47 nM as determined in SPR assay (Biacore T200).
Immobilized Human GITR Ligand Trimer, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human GITR, His Tag with the EC50 of 2.60ug/ml determined by ELISA (QC Test).
The purity of Human GITR Ligand (Trimer) is greater than 95% as determined by SEC-HPLC.
Human GITR Ligand (Trimer) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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