Human GITR Ligand/TNFSF18 Trimer Protein (FSF-HM218)
Catalog: FSF-HM218
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| Product Description | Recombinant Human GITR Ligand/TNFSF18 Trimer Protein is expressed from Expi293 with monomeric hFc tag at the N-terminal.It contains Gln50-Ser177. |
| Product Category | Recombinant Protein |
| Target | GITR Ligand/TNFSF18 Trimer |
| Synonyms | TNFSF18; AITRL; TL6; GITRL; GITR Ligand |
| Gene/Protein ID | Q9UNG2 |
| Species | Human |
| Exact Sequence | Gln50-Ser177 |
| Molecular Weight | The protein has a predicted MW of 70.2 kDa. Due to glycosylation, the protein migrates to 75-85 kDa based on Tris-Bis PAGE result. |
| Activity | The affinity constant of 4.47 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | N-monomeric hFc |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human GITR, His Tag captured on CM5 Chip via Anti-his antibody can bind Human GITR Ligand Trimer, hFc Tag with an affinity constant of 4.47 nM as determined in SPR assay (Biacore T200). |
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The purity of Human GITR Ligand (Trimer) is greater than 95% as determined by SEC-HPLC. |
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Human GITR Ligand (Trimer) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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