Name: Human GM-CSF Protein (GSF-HE001)
Brand: KactusBio
SKU: GSF-HE001-100UG
Price: 247.5 USD
Availability: InStock

Express System: E.coli
Product Tag: No Tag
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human GM-CSF at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human GM-CSF R alpha, hFc Tag with the EC50 of 10.3ng/ml determined by ELISA (QC Test). The affinity constant of 0.79nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Ala18-Glu144

Catalog #GSF-HE001

Size

100UG

500UG

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Product Description	Recombinant Human GM-CSF Protein is expressed from E.coli without tag.It contains Ala18-Glu144.
Product Background	Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts that functions as a cytokine. The pharmaceutical analogs of naturally occurring GM-CSF are called sargramostim and molgramostim.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	GM-CSF
Synonyms	CSF; CSF2; GMCSF; GM-CSF; MGC131935; MGC138897
Accession	P04141
Species	Human
Biotinylated	no
Amino Acid Range	Ala18-Glu144
Molecular Weight	The protein has a predicted MW of 14.6 kDa same as Bis-Tris PAGE result.
Activity	Immobilized Human GM-CSF at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human GM-CSF R alpha, hFc Tag with the EC50 of 10.3ng/ml determined by ELISA (QC Test). The affinity constant of 0.79nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	No Tag
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human GM-CSF R alpha, His Tag captured on CM5 Chip via anti-His antibody can bind Human GM-CSF, No Tag with an affinity constant of 0.79nM as determined in SPR assay (Biacore T200).
	Immobilized Human GM-CSF at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human GM-CSF R alpha, hFc Tag with the EC50 of 10.3ng/ml determined by ELISA (QC Test).
	Human GM-CSF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is 0.02-0.1 ng/mL (QC Test).
	The purity of Human GM-CSF is greater than 95% as determined by SEC-HPLC.