Name: Human GM-CSF R alpha Protein (GSF-HM1RA)
Brand: KactusBio
SKU: GSF-HM1RA-100UG
Price: 297.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human GM-CSF R alpha, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-GM-CSF R alpha Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA (QC Test). The affinity constant of 0.79nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Glu23-Gly320

Catalog #GSF-HM1RA

Size

100UG

500UG

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Product Description	Recombinant Human GM-CSF R alpha Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu23-Gly320.
Product Background	In the first trimester, the GM-CSF-R was found to be present on villous cytotrophoblast and all populations of extravillous trophoblast. Expression by villous syncytiotrophoblast was weak or absent, but this increased markedly by term. GM-CSF-R were also expressed by fetal Hofbauer cells within the mesenchyme of the chorionic villi and by uterine glandular epithelium and decidual macrophages within maternal decidua.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	GM-CSF R alpha
Synonyms	GM-CSF-R-alpha; GMCSFR-alpha; GMR-alpha; CSF2R; CSF2RY; CSF2RA; CDw116; CD116; CSF2RAX; CSF2RAY; CSF2RX; GMCSFR; GMR; SMDP4
Accession	P15509-1
Species	Human
Biotinylated	no
Amino Acid Range	Glu23-Gly320
Molecular Weight	The protein has a predicted MW of 35.3 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human GM-CSF R alpha, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-GM-CSF R alpha Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA (QC Test). The affinity constant of 0.79nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human GM-CSF R alpha , His Tag captured on CM5 Chip via anti-His antibody can bind Human GM-CSF, No Tag with an affinity constant of 0.79nM as determined in SPR assay (Biacore T200).
	Immobilized Human GM-CSF R alpha, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-GM-CSF R alpha Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA (QC Test).
	Immobilized Human GM-CSF, No Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human GM-CSF R alpha, His Tag with the EC50 of 1.0ug/ml determined by ELISA.
	Human GM-CSF R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human GM-CSF R alpha is greater than 95% as determined by SEC-HPLC.