Human gp130/CD130/IL-6 R beta-Protein (GP1-HM130)
Human gp130/CD130/IL-6 R beta-Protein (GP1-HM130)
Human gp130/CD130/IL-6 R beta-Protein (GP1-HM130)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-6 R alpha, hFc Tag at 2ug/ml (100ul/well) on the Human IL-6, No Tag precoated plate (5ug/ml). Dose response curve for Human gp130, His Tag with the EC50 of 49.6ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Glu23-Glu619
Catalog #GP1-HM130
Regular price $264.00
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Product Description

Recombinant Human gp130/CD130/IL-6 R beta-Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu23-Glu619.

Product Background

gp130 is a common signal transducing component of the functional receptor complexes for the interleukin (IL)-6 family of cytokines, ie, IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M, ciliary neurotrophic factor, and cardiotrophin-1. These cytokines exhibit pleiotropic biological activities in, for instance, immune, hematopoietic, and neural systems, and function in a redundant manner owing to the shared usage of gp130.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others
Protein
gp130/CD130/IL-6 R beta
Synonyms
gp130; CD130; IL-6 R beta; IL-6R-beta; IL-6RB; CDw130; IL6ST
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Glu23-Glu619
Molecular Weight
The protein has a predicted MW of 68.94 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human IL-6 R alpha, hFc Tag at 2ug/ml (100ul/well) on the Human IL-6, No Tag precoated plate (5ug/ml). Dose response curve for Human gp130, His Tag with the EC50 of 49.6ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human IL-6 R alpha, hFc Tag at 2ug/ml (100ul/well) on the Human IL-6, No Tag precoated plate (5ug/ml). Dose response curve for Human gp130, His Tag with the EC50 of 49.6ng/ml determined by ELISA (QC Test). Immobilized Human IL-6 R alpha, hFc Tag at 2ug/ml (100ul/well) on the Human IL-6, No Tag precoated plate (5ug/ml). Dose response curve for Human gp130, His Tag with the EC50 of 49.6ng/ml determined by ELISA (QC Test).
Human gp130 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human gp130 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human gp130 is greater than 95% as determined by SEC-HPLC. The purity of Human gp130 is greater than 95% as determined by SEC-HPLC.
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