Name: Human HGF R/c-MET Protein (MET-HM401)
Brand: KactusBio
SKU: MET-HM401-100UG
Price: 214.5 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human HGF R, His Tag at 2 ug/ml (100 ul/Well). Dose response curve for Human HGF, hFc Tag with the EC50 of 13.3 ng/ml determined by ELISA (QC test). See testing image for detail.
Amino Acid Range: Glu25-Thr932

Catalog #MET-HM401

Size

100UG

500UG

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Product Description	Recombinant Human HGF R/c-MET Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus; It contains Glu25-Thr932.
Product Background	c-Met, also called tyrosine-protein kinase Met or hepatocyte growth factor receptor (HGFR), is a protein that in humans is encoded by the MET gene. The protein possesses tyrosine kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	HGF R/c-MET
Synonyms	MET; oncogene MET; HGF R; HGF/SF receptor; AUTS9; cMET; Met (c-Met); RCCP2; SF receptor
Accession	P08581-1
Species	Human
Biotinylated	no
Amino Acid Range	Glu25-Thr932
Molecular Weight	The mature form of HGF R is a heterodimer which can be cleaved into α and β chain. The protein has a predicted MW of 32.5 kDa (α chain) and 72.1 kDa (β chain). Due to glycosylation, the protein migrates to 45-60 kDa (α subunit) and 80-100 kDa (β subunit) based on Bis-Tris PAGE result.
Activity	Immobilized Human HGF R, His Tag at 2 ug/ml (100 ul/Well). Dose response curve for Human HGF, hFc Tag with the EC50 of 13.3 ng/ml determined by ELISA (QC test). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human HGF R, His Tag at 2 ug/ml (100 ul/Well). Dose response curve for Human HGF, hFc Tag with the EC50 of 13.3 ng/ml determined by ELISA (QC test).
	Immobilized Human HGF R, His Tag at 5 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-HGF R Antibody, hFc Tag with the EC50 of 5.8 ng/ml determined by ELISA.
	Human HGF R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Human HGF, hFc Tag on ProA-Biosensor, can bind Human HGF R, His-Avi Tag with an affinity constant of 9.93 nM as determined in BLI assay (Gator® Prime).
	Loaded Anti-HGF R Antibody, hFc Tag on ProA-Biosensor, can bind Human HGF R, His-Avi Tag with an affinity constant of 0.79 nM as determined in BLI assay (Gator® Prime).
	The purity of Human HGF R is greater than 95% as determined by SEC-HPLC.