Human HGF R/c-MET Protein (MET-HM401)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Human HGF R, His Tag at 2μg/ml (100μl/Well). Dose response curve for Human HGF, hFc Tag with the EC50 of 0.16μg/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Glu25-Thr932

Catalog: MET-HM401

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Product Description Recombinant Human HGF R/c-MET Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.; It contains Glu25-Thr932.
Product Category Recombinant Protein
Target HGF R/c-MET
Synonyms MET; oncogene MET; HGF R; HGF/SF receptor; AUTS9; cMET; Met (c-Met); RCCP2; SF receptor
Gene/Protein ID P08581-1
Species Human
Exact Sequence Glu25-Thr932
Molecular Weight The protein has a predicted MW of 32.5 kDa (α chain) and 72.1 kDa (β chain). Due to glycosylation, the protein migrates to 45-55 kDa and 80-85 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human HGF R, His Tag at 2μg/ml (100μl/Well). Dose response curve for Human HGF, hFc Tag with the EC50 of 0.16μg/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human HGF R, His Tag at 2μg/ml (100μl/Well). Dose response curve for Human HGF, hFc Tag with the EC50 of 0.16μg/ml determined by ELISA.
Immobilized Human HGF R, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-HGF R Antibody, hFc Tag with the EC50 of 24.8ng/ml determined by ELISA.
The purity of Human HGF R is greater than 95% as determined by SEC-HPLC.
Human HGF R on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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