Human HGF R/c-MET Protein (MET-HM401)
Human HGF R/c-MET Protein (MET-HM401)
Human HGF R/c-MET Protein (MET-HM401)
Human HGF R/c-MET Protein (MET-HM401)
Human HGF R/c-MET Protein (MET-HM401)
Human HGF R/c-MET Protein (MET-HM401)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
  • Activity: Immobilized Human HGF R, His Tag at 5 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-HGF R Antibody, hFc Tag with the EC50 of 5.8 ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Glu25-Thr932
Catalog #MET-HM401
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Product Description
 Recombinant Human HGF R/c-MET Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus; It contains Glu25-Thr932.
Product Background
 c-Met, also called tyrosine-protein kinase Met or hepatocyte growth factor receptor (HGFR), is a protein that in humans is encoded by the MET gene. The protein possesses tyrosine kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1.
Product Category
 Recombinant Protein
Target
 HGF R/c-MET
Synonyms
 MET; oncogene MET; HGF R; HGF/SF receptor; AUTS9; cMET; Met (c-Met); RCCP2; SF receptor
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Glu25-Thr932
Molecular Weight
 The mature form of HGF R is a heterodimer which can be cleaved into α and β chain. The protein has a predicted MW of 32.5 kDa (α chain) and 72.1 kDa (β chain). Due to glycosylation, the protein migrates to 45-60 kDa (α subunit) and 80-100 kDa (β subunit) based on Bis-Tris PAGE result.
Activity
 Immobilized Human HGF R, His Tag at 5 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-HGF R Antibody, hFc Tag with the EC50 of 5.8 ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-His-Avi
Express System
 HEK293
Purity
 >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human HGF R, His Tag at 5 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-HGF R Antibody, hFc Tag with the EC50 of 5.8 ng/ml determined by ELISA.
Immobilized Human HGF R, His Tag at 1 ug/ml (100 ul/Well). Dose response curve for Human HGF, hFc Tag with the EC50 of 26.3 ng/ml determined by ELISA (QC test).
Human HGF R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human HGF R is greater than 95% as determined by SEC-HPLC.
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