Human HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV) Monomer Protein (MHC-HE013)

  • Express System: E.coli
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Exact Sequence: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and NLVPMVATV peptide

Catalog: MHC-HE013

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Product Description Recombinant Human HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus.It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and NLVPMVATV peptide.

Product Background

Human cytomegalovirus (CMV), a β-herpes virus with a double-stranded DNA, infects a wide variety of cells and establishes latency in the host. CMVpp65, a tegument protein of the herpes virus CMV, is the main viral antigen found in peripheral blood mononuclear cells (PBMCs) after viral infection and may activate cell-mediated immunity, accounting for 70-90% of the cytotoxic CD8+ T cells' (CTLs) response to CMV. Among the pp65-derived CTL epitope peptides, the 9-mer peptide 495NLVPMVATV503 (CMVpp65 495-503 peptide) is the most immunogenic T cell epitope predominantly displayed on HLA-A*02:01, the most common MHC-I allele in the population.

Product Category

Recombinant Protein

Target

HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV)
MHC Form Monomer
Allele HLA-A*02:01

Gene/Protein ID

A0A140T913(HLA-A*02:01)&P61769(B2M)&NLVPMVATV

Species

Human

Exact Sequence

Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and NLVPMVATV peptide
Molecular Weight The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Tris-Bis PAGE result.

Product Tag

C-His-Avi

Express System

E.coli
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Liquid
Shipping Shipped with dry ice.
Formulation Supplied as 0.22 um filtered solution in 20mM Tris, 200mM NaCl (pH 8.0).
Stability And Storage Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
The purity of Human HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV) Monomer is greater than 95% as determined by SEC-HPLC.
Human HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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