Human HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV) Monomer Protein (MHC-HE013)
Human HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV) Monomer Protein (MHC-HE013)
Human HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV) Monomer Protein (MHC-HE013)
  • Express System: E.coli
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV) Monomer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 7.3ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and NLVPMVATV peptide
Catalog #MHC-HE013
Regular price $310.00
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Product Description
Recombinant Human HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus.It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and NLVPMVATV peptide.
Product Background
Human cytomegalovirus (CMV), a β-herpes virus with a double-stranded DNA, infects a wide variety of cells and establishes latency in the host. CMVpp65, a tegument protein of the herpes virus CMV, is the main viral antigen found in peripheral blood mononuclear cells (PBMCs) after viral infection and may activate cell-mediated immunity, accounting for 70-90% of the cytotoxic CD8+ T cells' (CTLs) response to CMV. Among the pp65-derived CTL epitope peptides, the 9-mer peptide 495NLVPMVATV503 (CMVpp65 495-503 peptide) is the most immunogenic T cell epitope predominantly displayed on HLA-A*02:01, the most common MHC-I allele in the population.
Product Category
Recombinant Protein
Target
HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV)
Synonyms
CMVpp65; NLVPMVATV
MHC Form
Monomer
Allele
HLA-A*02:01
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and NLVPMVATV peptide
Molecular Weight
The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
Activity
Immobilized Human HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV) Monomer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 7.3ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His-Avi
Express System
E.coli
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22 um filtered solution in 20mM Tris, 200mM NaCl (pH 8.0).
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV) Monomer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 7.3ng/ml determined by ELISA.
Human HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV) Monomer is greater than 95% as determined by SEC-HPLC.

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