Name: Human HLA-B*15:01&B2M&SARS-CoV-2 epitope (NQKLIANQF) Monomer Protein (MHC-HM448)
Brand: KactusBio
SKU: MHC-HM448-100UG
Price: 341.0 USD
Availability: InStock

Human HLA-B*15:01&B2M&SARS-CoV-2 epitope (NQKLIANQF) Monomer Protein (MHC-HM448)

Human HLA-B*15:01&B2M&SARS-CoV-2 epitope (NQKLIANQF) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human HLA-B*15:01&B2M&SARS-CoV-2 epitope (NQKLIANQF) Monomer is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Amino Acid Range: Gly21-Thr301(HLA-B*15:01), Ile21-Met119(B2M) and NQKLIANQF peptide

Catalog #MHC-HM448

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Human HLA-B*15:01&B2M&SARS-CoV-2 epitope (NQKLIANQF) Monomer Protein (MHC-HM448) Summary

Product Description	Recombinant Human HLA-B15:01&B2M&SARS-CoV-2 epitope (NQKLIANQF) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Gly21-Thr301(HLA-B15:01), Ile21-Met119(B2M) and NQKLIANQF peptide.
Product Background	HLA-B15:01 is strongly associated with asymptomatic infection with SARS-CoV-2 and is likely to be involved in the mechanism underlying early viral clearance. T cells from pre-pandemic individuals carrying HLA-B15:01 were reactive to the immunodominant SARS-CoV-2 S-derived peptide NQKLIANQF, and 100% of the reactive cells displayed memory phenotype.
Product Category	Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs

HLA-B*15:01&B2M&SARS-CoV-2 epitope (NQKLIANQF) Specifications

Protein	HLA-B*15:01&B2M&SARS-CoV-2 epitope (NQKLIANQF)
Synonyms	MHC; SARS-CoV-2 epitope;
MHC Form	Monomer
Allele	HLA-B*15:01
Accession	AAA53258.1(HLA-B*15:01)&P61769(B2M)&NQKLIANQF
Species	Human
Biotinylated	no
Amino Acid Range	Gly21-Thr301(HLA-B*15:01), Ile21-Met119(B2M) and NQKLIANQF peptide
Molecular Weight	The protein has a predicted MW of 50.50 kDa. Due to glycosylation, the protein migrates to 52-68 kDa based on Bis-Tris PAGE result.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Human HLA-B*15:01&B2M&SARS-CoV-2 epitope (NQKLIANQF) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human HLA-B*15:01&B2M&SARS-CoV-2 epitope (NQKLIANQF) Monomer is greater than 95% as determined by SEC-HPLC.