Product Description |
Recombinant Human HPN/Hepsin Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Arg45-Leu417. |
Product Background |
Home parenteral nutrition (HPN) has been proposed as the treatment of choice in patients suffering from intestinal failure (IF) and has been claimed to improve survival and quality of life either in patients with benign disorders or even in those with malignancies. HPN seems to have beneficial effect but it should be considered with caution by the physicians who should take into account the indications of each patient to receive parenteral nutrition, the underlying disease and prognosis and the access of each patient to home care services. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Protein |
HPN/Hepsin |
Synonyms |
Serine protease hepsin; TMPRSS1; Hepsin |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Arg45-Leu417 |
Molecular Weight |
The protein has a predicted MW of 42 kDa. Due to the protein cleaved into the following two chains, so it migrates to 18-22 kDa and 30 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human HPN, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-HPN Antibody, hFc Tag with the EC50 of 14.9ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 20mM NaAc,150mM NaCl (pH 5.0). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in 20mM NaAc,150mM NaCl (pH 5.0). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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